The sperm of mammals are very sensitive to temperature fluctuations with species and individual differences. All materials having contact with the sperm should be preheated to this temperature. The handling temperature during semen evaluation is usually 37 ☌. Busch and Fischer (2007) suggested that the collected semen must be evaluated within 10 min after collection, without describing the influence of time on semen quality. In other studies the time interval between collection and evaluation was 2–3 min, after 20 min or for the entire evaluation within one hour after collection, while concentration, pH and volume were examined immediately after collection. Usually the semen evaluation takes place directly after the collection.
Ī standard protocol for evaluation of goat semen does not exist. In human medicine, guidelines for semen collection, handling and evaluation of the ejaculates are described. Therefore, the handling conditions must be kept as constant as possible during the evaluation. These are used to determine the suitability of an ejaculate for further use and to establish an insemination dose. In order to obtain comparable results, semen samples should always be examined within 10 min after collection.ĭifferent parameters are assessed as part of the semen analysis including in general volume, color, consistency, impurities as well as semen concentration, motility, vitality and morphology. Raw goat semen can tolerate room temperatures for at least 10 min without impacting overall semen quality. ConclusionĮxamination time point was identified as factor strongly influencing raw peacock buck semen after collection. ResultsĮxamination time point had a significant influence on raw sperm viability ( p 0.05), motility ( p > 0.05), with the exception of fast moving sperm ( p = 0.04), or on semen pH ( p > 0.05). Here we examined the effects of two handling temperatures (20 ☌, 37 ☌) and various examination time points (3–30 min) after semen collection. The aim of this study was to analyse two different factors affecting buck sperm quality in the post-collection period prior to adding the extender. Different parameters are assessed as part of the semen analysis but a standard protocol for evaluation of goat semen is still missing.
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